125i labeled progesterone Search Results


progesterone  (Tecan Systems)
94
Tecan Systems progesterone
Progesterone, supplied by Tecan Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/125i+labeled+progesterone/Progesterone+Saliva+ELISA/pmc04742768-179-13-14
Average 94 stars, based on 1 article reviews
progesterone - by Bioz Stars, 2026-11
94/100 stars
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progesterone  (Valiant Co Ltd)
96
Valiant Co Ltd progesterone
(A) Schematic of the initial steps in the steroidogenic pathway: (i) Mitochondrial cholesterol import is mediated by the intermembrane space shuttle STAR. (ii) Cholesterol is converted to pregnenolone/P5 by CYP11A1 in the mitochondrial matrix. (iii) P5 is further converted to <t>progesterone/P4</t> by 3β-hydroxysteroid dehydrogenase (HSD3B) at the endoplasmic reticulum. (B) Dose-response analysis of progesterone production in wild-type MA-10 and TSPO-null MA-10: Tspo Δ/Δ cells stimulated with Bt 2 cAMP. Increasing concentrations of 19-Atriol reduced P4 output in both genotypes (*p<0.05) with comparable potency and maximal inhibition, indicating that its pharmacological action is independent of TSPO. (C) Immunoblot analysis of STAR and CYP11A1 expression following treatment with 19-Atriol (0,10 and 100 μM). TSPO was absent in MA-10: Tspo Δ/Δ cells, validating the knockout. Neither STAR nor CYP11A1 protein abundance was altered by 19-Atriol in either genotype (ACTB served as a loading control). (D) Dose-response analysis of progesterone production in wild-type MA-10 and TSPO-null MA-10: Tspo Δ/Δ cells with supplementation of 22R-HC in the absence of stimulation. 19-Atriol continued to inhibit P4 production in both genotypes (*p<0.05), indicating that the observed inhibitory effect occurs downstream of cholesterol import.
Progesterone, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/125i+labeled+progesterone/Progesterone-water+soluble/bio_rxiv__2025__09__25__678677-155-11-12
Average 96 stars, based on 1 article reviews
progesterone - by Bioz Stars, 2026-11
96/100 stars
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125i labeled progesterone  (Valiant Co Ltd)
93
Valiant Co Ltd 125i labeled progesterone
(A) Schematic of the initial steps in the steroidogenic pathway: (i) Mitochondrial cholesterol import is mediated by the intermembrane space shuttle STAR. (ii) Cholesterol is converted to pregnenolone/P5 by CYP11A1 in the mitochondrial matrix. (iii) P5 is further converted to <t>progesterone/P4</t> by 3β-hydroxysteroid dehydrogenase (HSD3B) at the endoplasmic reticulum. (B) Dose-response analysis of progesterone production in wild-type MA-10 and TSPO-null MA-10: Tspo Δ/Δ cells stimulated with Bt 2 cAMP. Increasing concentrations of 19-Atriol reduced P4 output in both genotypes (*p<0.05) with comparable potency and maximal inhibition, indicating that its pharmacological action is independent of TSPO. (C) Immunoblot analysis of STAR and CYP11A1 expression following treatment with 19-Atriol (0,10 and 100 μM). TSPO was absent in MA-10: Tspo Δ/Δ cells, validating the knockout. Neither STAR nor CYP11A1 protein abundance was altered by 19-Atriol in either genotype (ACTB served as a loading control). (D) Dose-response analysis of progesterone production in wild-type MA-10 and TSPO-null MA-10: Tspo Δ/Δ cells with supplementation of 22R-HC in the absence of stimulation. 19-Atriol continued to inhibit P4 production in both genotypes (*p<0.05), indicating that the observed inhibitory effect occurs downstream of cholesterol import.
125i Labeled Progesterone, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/125i+labeled+progesterone/Progesterone+Coated+Tube+RIA+Kit/pmc12896842-175-12-21
Average 93 stars, based on 1 article reviews
125i labeled progesterone - by Bioz Stars, 2026-11
93/100 stars
  Buy from Supplier

Image Search Results


(A) Schematic of the initial steps in the steroidogenic pathway: (i) Mitochondrial cholesterol import is mediated by the intermembrane space shuttle STAR. (ii) Cholesterol is converted to pregnenolone/P5 by CYP11A1 in the mitochondrial matrix. (iii) P5 is further converted to progesterone/P4 by 3β-hydroxysteroid dehydrogenase (HSD3B) at the endoplasmic reticulum. (B) Dose-response analysis of progesterone production in wild-type MA-10 and TSPO-null MA-10: Tspo Δ/Δ cells stimulated with Bt 2 cAMP. Increasing concentrations of 19-Atriol reduced P4 output in both genotypes (*p<0.05) with comparable potency and maximal inhibition, indicating that its pharmacological action is independent of TSPO. (C) Immunoblot analysis of STAR and CYP11A1 expression following treatment with 19-Atriol (0,10 and 100 μM). TSPO was absent in MA-10: Tspo Δ/Δ cells, validating the knockout. Neither STAR nor CYP11A1 protein abundance was altered by 19-Atriol in either genotype (ACTB served as a loading control). (D) Dose-response analysis of progesterone production in wild-type MA-10 and TSPO-null MA-10: Tspo Δ/Δ cells with supplementation of 22R-HC in the absence of stimulation. 19-Atriol continued to inhibit P4 production in both genotypes (*p<0.05), indicating that the observed inhibitory effect occurs downstream of cholesterol import.

Journal: bioRxiv

Article Title: Target validation uncouples TSPO from 19-Atriol-mediated inhibition of steroidogenesis and reveals true enzymatic targets

doi: 10.1101/2025.09.25.678677

Figure Lengend Snippet: (A) Schematic of the initial steps in the steroidogenic pathway: (i) Mitochondrial cholesterol import is mediated by the intermembrane space shuttle STAR. (ii) Cholesterol is converted to pregnenolone/P5 by CYP11A1 in the mitochondrial matrix. (iii) P5 is further converted to progesterone/P4 by 3β-hydroxysteroid dehydrogenase (HSD3B) at the endoplasmic reticulum. (B) Dose-response analysis of progesterone production in wild-type MA-10 and TSPO-null MA-10: Tspo Δ/Δ cells stimulated with Bt 2 cAMP. Increasing concentrations of 19-Atriol reduced P4 output in both genotypes (*p<0.05) with comparable potency and maximal inhibition, indicating that its pharmacological action is independent of TSPO. (C) Immunoblot analysis of STAR and CYP11A1 expression following treatment with 19-Atriol (0,10 and 100 μM). TSPO was absent in MA-10: Tspo Δ/Δ cells, validating the knockout. Neither STAR nor CYP11A1 protein abundance was altered by 19-Atriol in either genotype (ACTB served as a loading control). (D) Dose-response analysis of progesterone production in wild-type MA-10 and TSPO-null MA-10: Tspo Δ/Δ cells with supplementation of 22R-HC in the absence of stimulation. 19-Atriol continued to inhibit P4 production in both genotypes (*p<0.05), indicating that the observed inhibitory effect occurs downstream of cholesterol import.

Article Snippet: Briefly, supernatants were incubated overnight at 4 °C with 125 I-labeled progesterone (MP Biomedicals) and an anti-progesterone antibody ( ) for competitive binding.

Techniques: Inhibition, Western Blot, Expressing, Knock-Out, Quantitative Proteomics, Control